Mutagenesis:Article Title: Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Q5 Site-Directed Mutagenesis Kit New England Biolabs E0552 PierceTM High Sensitivity Streptavidin-HRP Thermo Fisher Scientific 21130 NEBuilder HiFi DNA Gibson Assembly Master Mix New England Biolabs E2621 p24 ZeptoMetrix Kit Sigma 0801111 Experimental models: Cell lines HEK293T cells ATCC CRL-11268 Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Strain: Crl:CD(SD) Oligonucleotides See Table S2 for oligonucleotides used for site-directed mutagenesis This paper N/A Recombinant DNA CamKII-LGI1-pHluorin This paper Addgene Plasmid #185537 CamKII-LGI1-S473L-pHluorin This paper Addgene Plasmid #185540 CamKII-LGI1-Y433A-pHluorin This paper Addgene Plasmid #185541 CamKII-LGI1-R474Q-pHluorin This paper Addgene Plasmid #185542 CamKII-LGI1-T380A-pHluorin This paper Addgene Plasmid #185543 CamKII-LGI1-E383A-pHluorin This paper Addgene Plasmid #185544 CamKII-LGI1-C200R-pHluorin This paper Addgene Plasmid #185545 CamKII-LGI1 (no tags) This paper N/A vGlut-pH Gift from Timothy A. Ryan34 N/A vGlut-mOrange-2 Hoppa et al., 201280 N/A iGluSnFR3 Aggarwal et al.58 Addgene Plasmid #178330 CMV-mRuby-synapsin Gift from Timothy A. Ryan Addgene Plasmid #187896 pKanCMV-mRuby3-18aa-actin Bajar et al.86 Addgene plasmid #74255 pLKO-synapsin-BFP Gift from Timothy A. Ryan Addgene Plasmid #191567 pLKO-empty-mTagBFP Gift from Timothy A. Ryan Addgene Plasmid #191566 CamKII-ADAM23-pHluorin This paper Addgene Plasmid #185539 pGP-AAV-syn-jGCaMP8f-WPRE Zhang et al.55 Addgene Plasmid #162376 CamKII-LGI1-pHmScarlet This paper Addgene Plasmid #185538 CamKII-LGI1-R474Q-pHmScarlet This paper N/A CamKII-LGI1-T380A-pHmScarlet This paper N/A CamKII-LGI1-E383A-pHmScarlet This paper N/A pCAGGS-SNAP25DC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-SNAP29DC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-Stx1ADC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-Stx4DC-IRES-mCherry Ibata et al., 201943 N/A CMV-IgK-pHluorin-TM-mRuby This paper Addgene Plasmid #185546 ER-pHluorin de Juan-Sanz et al., 201736 N/A FSW-HRP-V5-LRRTM2 Loh et al.52 Addgene Plasmid #82537 FSW-HRP-V5-LRRTM1 Loh et al.52 Addgene Plasmid #82536 pAAV-CaMKII-Archon1-EGFP Piatkevich et al.60 Addgene Plasmid #108417 hSyn-NPY-pHluorin Gift from Ruud F Toonen Persoon et al. 87 N/A VAMP2-pHmScarlet Gift from Pingyong Xu Liu et al.54 Addgene plasmid #166890 CMV-mRuby-synapsin Gift from Timothy A. Ryan Addgene Plasmid #187896 pLKO-synapsin-BFP Gift from Timothy A. Ryan Addgene Plasmid #191567 pLKO-empty-mTagBFP Gift from Timothy A. Ryan Addgene Plasmid #191566 (Continued on next page) Cell Reports 43, 114186, May 28, 2024 21 Article ll OPEN ACCESS
Recombinant:Article Title: Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Q5 Site-Directed Mutagenesis Kit New England Biolabs E0552 PierceTM High Sensitivity Streptavidin-HRP Thermo Fisher Scientific 21130 NEBuilder HiFi DNA Gibson Assembly Master Mix New England Biolabs E2621 p24 ZeptoMetrix Kit Sigma 0801111 Experimental models: Cell lines HEK293T cells ATCC CRL-11268 Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Strain: Crl:CD(SD) Oligonucleotides See Table S2 for oligonucleotides used for site-directed mutagenesis This paper N/A Recombinant DNA CamKII-LGI1-pHluorin This paper Addgene Plasmid #185537 CamKII-LGI1-S473L-pHluorin This paper Addgene Plasmid #185540 CamKII-LGI1-Y433A-pHluorin This paper Addgene Plasmid #185541 CamKII-LGI1-R474Q-pHluorin This paper Addgene Plasmid #185542 CamKII-LGI1-T380A-pHluorin This paper Addgene Plasmid #185543 CamKII-LGI1-E383A-pHluorin This paper Addgene Plasmid #185544 CamKII-LGI1-C200R-pHluorin This paper Addgene Plasmid #185545 CamKII-LGI1 (no tags) This paper N/A vGlut-pH Gift from Timothy A. Ryan34 N/A vGlut-mOrange-2 Hoppa et al., 201280 N/A iGluSnFR3 Aggarwal et al.58 Addgene Plasmid #178330 CMV-mRuby-synapsin Gift from Timothy A. Ryan Addgene Plasmid #187896 pKanCMV-mRuby3-18aa-actin Bajar et al.86 Addgene plasmid #74255 pLKO-synapsin-BFP Gift from Timothy A. Ryan Addgene Plasmid #191567 pLKO-empty-mTagBFP Gift from Timothy A. Ryan Addgene Plasmid #191566 CamKII-ADAM23-pHluorin This paper Addgene Plasmid #185539 pGP-AAV-syn-jGCaMP8f-WPRE Zhang et al.55 Addgene Plasmid #162376 CamKII-LGI1-pHmScarlet This paper Addgene Plasmid #185538 CamKII-LGI1-R474Q-pHmScarlet This paper N/A CamKII-LGI1-T380A-pHmScarlet This paper N/A CamKII-LGI1-E383A-pHmScarlet This paper N/A pCAGGS-SNAP25DC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-SNAP29DC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-Stx1ADC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-Stx4DC-IRES-mCherry Ibata et al., 201943 N/A CMV-IgK-pHluorin-TM-mRuby This paper Addgene Plasmid #185546 ER-pHluorin de Juan-Sanz et al., 201736 N/A FSW-HRP-V5-LRRTM2 Loh et al.52 Addgene Plasmid #82537 FSW-HRP-V5-LRRTM1 Loh et al.52 Addgene Plasmid #82536 pAAV-CaMKII-Archon1-EGFP Piatkevich et al.60 Addgene Plasmid #108417 hSyn-NPY-pHluorin Gift from Ruud F Toonen Persoon et al. 87 N/A VAMP2-pHmScarlet Gift from Pingyong Xu Liu et al.54 Addgene plasmid #166890 CMV-mRuby-synapsin Gift from Timothy A. Ryan Addgene Plasmid #187896 pLKO-synapsin-BFP Gift from Timothy A. Ryan Addgene Plasmid #191567 pLKO-empty-mTagBFP Gift from Timothy A. Ryan Addgene Plasmid #191566 (Continued on next page) Cell Reports 43, 114186, May 28, 2024 21 Article ll OPEN ACCESS
Plasmid Preparation:Article Title: Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Q5 Site-Directed Mutagenesis Kit New England Biolabs E0552 PierceTM High Sensitivity Streptavidin-HRP Thermo Fisher Scientific 21130 NEBuilder HiFi DNA Gibson Assembly Master Mix New England Biolabs E2621 p24 ZeptoMetrix Kit Sigma 0801111 Experimental models: Cell lines HEK293T cells ATCC CRL-11268 Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Strain: Crl:CD(SD) Oligonucleotides See Table S2 for oligonucleotides used for site-directed mutagenesis This paper N/A Recombinant DNA CamKII-LGI1-pHluorin This paper Addgene Plasmid #185537 CamKII-LGI1-S473L-pHluorin This paper Addgene Plasmid #185540 CamKII-LGI1-Y433A-pHluorin This paper Addgene Plasmid #185541 CamKII-LGI1-R474Q-pHluorin This paper Addgene Plasmid #185542 CamKII-LGI1-T380A-pHluorin This paper Addgene Plasmid #185543 CamKII-LGI1-E383A-pHluorin This paper Addgene Plasmid #185544 CamKII-LGI1-C200R-pHluorin This paper Addgene Plasmid #185545 CamKII-LGI1 (no tags) This paper N/A vGlut-pH Gift from Timothy A. Ryan34 N/A vGlut-mOrange-2 Hoppa et al., 201280 N/A iGluSnFR3 Aggarwal et al.58 Addgene Plasmid #178330 CMV-mRuby-synapsin Gift from Timothy A. Ryan Addgene Plasmid #187896 pKanCMV-mRuby3-18aa-actin Bajar et al.86 Addgene plasmid #74255 pLKO-synapsin-BFP Gift from Timothy A. Ryan Addgene Plasmid #191567 pLKO-empty-mTagBFP Gift from Timothy A. Ryan Addgene Plasmid #191566 CamKII-ADAM23-pHluorin This paper Addgene Plasmid #185539 pGP-AAV-syn-jGCaMP8f-WPRE Zhang et al.55 Addgene Plasmid #162376 CamKII-LGI1-pHmScarlet This paper Addgene Plasmid #185538 CamKII-LGI1-R474Q-pHmScarlet This paper N/A CamKII-LGI1-T380A-pHmScarlet This paper N/A CamKII-LGI1-E383A-pHmScarlet This paper N/A pCAGGS-SNAP25DC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-SNAP29DC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-Stx1ADC-IRES-mCherry Ibata et al., 201943 N/A pCAGGS-Stx4DC-IRES-mCherry Ibata et al., 201943 N/A CMV-IgK-pHluorin-TM-mRuby This paper Addgene Plasmid #185546 ER-pHluorin de Juan-Sanz et al., 201736 N/A FSW-HRP-V5-LRRTM2 Loh et al.52 Addgene Plasmid #82537 FSW-HRP-V5-LRRTM1 Loh et al.52 Addgene Plasmid #82536 pAAV-CaMKII-Archon1-EGFP Piatkevich et al.60 Addgene Plasmid #108417 hSyn-NPY-pHluorin Gift from Ruud F Toonen Persoon et al. 87 N/A VAMP2-pHmScarlet Gift from Pingyong Xu Liu et al.54 Addgene plasmid #166890 CMV-mRuby-synapsin Gift from Timothy A. Ryan Addgene Plasmid #187896 pLKO-synapsin-BFP Gift from Timothy A. Ryan Addgene Plasmid #191567 pLKO-empty-mTagBFP Gift from Timothy A. Ryan Addgene Plasmid #191566 (Continued on next page) Cell Reports 43, 114186, May 28, 2024 21 Article ll OPEN ACCESS
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